FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l03n02_g6.352000000a8a6c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l03n02_g6.352000000a8a6c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44304512
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG500150.018.4378781
GTATAGG151450.015.5909571
TATTGGG514250.015.548332
TATACTG209150.015.5368995
TGCGTTC404350.015.5189019
TAGACTG125550.015.1737785
GGTGCGT439250.014.7946137
GTATATA569050.014.0263441
GTGCGTT456400.014.0092548
ATTGGGT548100.013.9293383
GTATAAG180000.013.9098931
TATACAG199950.013.6619455
TACACTG158350.013.1708125
GTACAGG131750.013.1621821
TTGGGTG553950.013.130274
GTGTAGG151700.013.0284711
GTCTATA198800.012.9768461
GTCCTAG141000.012.7030471
TATACCG174850.012.6615345
GTATTAC181050.012.5958771