FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l03n02_g5.352000000a8a1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l03n02_g5.352000000a8a1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40338495
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG137750.016.4166645
TATACTG209350.015.2725545
GTATTGG332750.015.2630981
GTATAGG155500.014.45152951
TACACCG156300.014.3771165
TATACCG129950.014.0751825
GTCTATA173650.013.4882351
TACACTG170500.013.1239975
GTATATA455700.013.1050681
ACTGATC274350.013.0033118
TATACAG205550.012.9200425
GTACAGG173550.012.7842511
TATTGGG359750.012.7042082
CTATACT208400.012.6522444
TATAGAC188400.012.3815663
CCTACAC166250.012.3451693
CTAAGAC156900.012.3238523
GGTATAG164400.012.310931
AGTACCG114350.012.2146425
CCTATAC146750.012.107893
Produced by FastQC (version 0.11.2)