FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l03n01_g7.351000000a8ae6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l03n01_g7.351000000a8ae6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59227649
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG180600.018.2273485
TACACCG246250.017.6310425
GTATTGG417900.017.2956641
TATACTG264500.015.9296415
TATAGAC236850.015.8636493
GTCTATA213450.015.5173311
TACACAG247700.015.0348155
GTATAGG203700.014.9536571
TACACTG257000.014.8604155
CTACACC388550.014.6826034
GTATATA546250.014.4409921
GGTATAG230650.014.3807771
TATACAG268000.014.3745465
AGACCTA277350.014.352386
TATTGGG432100.014.279892
CTATACT265400.013.9425924
GTGTAGG269400.013.8821761
GTGTAGA269700.013.8491151
GTATAAG171750.013.8065191
GTCTAGG195750.013.7645411