FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l03n01_g5.351000000a8a1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l03n01_g5.351000000a8a1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40338495
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG134550.016.9816555
TATACTG206100.015.6729085
GTATTGG335800.015.2129281
GTACCGT109950.014.1708966
TATACAG215900.013.6413615
TATACCG131200.013.5049725
ACTGTGC179650.013.4588238
GTATAAG152100.013.3721011
TACACCG153950.013.2680625
TATAGAC184450.013.1854643
GTGTAGA190300.013.1100861
TACACTG175600.012.9848055
TACCGTC102250.012.9149957
GTCTATA171250.012.7924991
AGTACCG120600.012.7225425
GTGTAGG170050.012.6312661
ACCGTCC147250.012.6135698
GTATAGG150450.012.57117751
GTCTAGG131900.012.5377471
GTATATA466200.012.5377011
Produced by FastQC (version 0.11.2)