FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l02n02_r9.352000000a8b28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l02n02_r9.352000000a8b28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35914158
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG188100.021.1626661
CCTATAC196450.017.6034893
CTATACT250950.017.3790364
TATACTG204350.016.4134885
TATAGGG181150.015.0516852
TAGACTG118300.014.7786215
CCCTATA190550.014.5086032
GTGTAGG157400.014.4559281
TACACTG167700.014.0513955
GTATAAG158200.013.9624511
CTACACT166700.013.5941794
GTATTGG198500.013.5926141
TATACCG110550.013.4940325
TATACTA343350.013.4769845
GTATAGA170650.013.4448521
GTATATA431450.013.1788911
GTCCTAG127650.013.1361791
TACACAG153400.013.1004375
TATACAG201250.013.0544955
ACTGTGC160850.013.0252838