FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l02n02_r8.352000000a8ac9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l02n02_r8.352000000a8ac9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38936471
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG305600.031.4302561
TATAGGG328650.026.0890052
CCTATAC366800.025.4872913
TAGGGAT340350.024.0382464
CTATACT419650.024.0244564
ATAGGGA339400.023.7938633
CCCTATA366900.023.1749742
AGGGATG391500.021.35885
TCCCTAT382650.021.1537781
TACTAAC390400.020.2019427
TATACTA517300.019.865985
ATACTAA453900.018.8097616
CTAACCA447450.018.146349
ATGGCAC410350.017.7607969
GGATGGC431750.017.3647887
GACGTTA204200.017.25702520-21
GGGATGG465100.016.824526
TATACTG221850.016.2760935
CGTTAGG214800.016.14008322-23
GATGGCA457650.015.9875568