FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l02n02_r10.352000000a8b92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l02n02_r10.352000000a8b92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36983899
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG191050.019.094321
CCTATAC192850.016.7731913
TATACTG204250.016.2815765
CTATACT237750.015.6659244
TACACTG169800.014.4648295
TACACCG128400.014.2817945
ACTGTGC159000.013.8039928
GTGTAGG173100.013.5007551
GTATATA451750.013.3745421
CTACACT171100.013.0777014
TAGACTG114850.012.9885045
TATAGGG184250.012.9669952
GTCCTAG136400.012.9196131
TATACAG208950.012.7322795
TATACTA328250.012.6637935
TATACCG118900.012.4661755
CCCTATA178350.012.4638322
GTCTAGG114450.012.45074751
CTACACC204400.012.4114034
GTATTGG186000.012.3345751