FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l02n02_g4.352000000a8d40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l02n02_g4.352000000a8d40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42106442
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG401750.016.3278471
TATTGGG400300.014.5713162
TACACCG179950.013.7809595
TAGACTG117400.013.4752525
ATTGGGT415550.013.0195573
GTATAAG133900.012.9480221
TATACTG184900.012.7183025
GTATATA400900.012.6658061
GTCTAGG138250.012.6436891
GTATAGG136000.012.6433111
TGCGTTC363850.012.4953329
GTGTAGG174750.012.4219621
GTATTAG153050.012.2589971
TATACCG146800.012.0710095
TATACAG176550.011.8398385
CTACACC262000.011.659264
GTATAGA181600.011.5349021
CCTATAC142700.011.4506533
TACACTG153950.011.4486695
TTGGGTG457550.011.3486174