FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l02n02_g3.352000000a8cba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l02n02_g3.352000000a8cba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49446458
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG159900.015.7767155
TATACTG275250.014.7919025
GTATTGG376550.014.7721191
TACACCG176050.014.14053255
GTATAGG183050.013.5978471
TATACAG258050.013.4028775
GTCTAGG151750.012.7401431
GTATATA610750.012.5374761
TATACCG158450.012.5329865
GTATAAG196400.012.5284361
GTCTATA223700.012.5071361
ACTGTGC220800.012.436528
TACACTG215900.012.3006965
CTACACC283200.012.2964934
GTGTAGG220800.012.2841651
ACTGCGC124600.012.2393138
CTATACT267000.012.1884884
GTACAGG201200.012.1823271
TACACAG218900.012.1538195
TATTGGG399500.012.1516612
Produced by FastQC (version 0.11.2)