FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l02n02_g2.352000000a8c5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l02n02_g2.352000000a8c5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36404559
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG190100.016.244685
TAGACTG120300.015.4415555
GTATTGG296800.015.2845261
TACACCG140150.015.0172545
GTATAGG136150.014.8629311
GTGTAGG159000.014.848131
TACACTG155350.014.1595565
TATACAG186650.013.8468455
GTATAAG139400.013.8008161
GTCTATA157950.013.5634261
GTATATA426000.013.336261
GTACAGG154150.013.0349481
CTATACT191300.012.9390414
GTCTATG151850.012.7631491
GTACCGT97150.012.5191976
TATACCG117650.012.4780435
TCTACAC164800.012.4521683
AGTACCG102300.012.4462665
GTGTATG216950.012.4365861
CTACACT158450.012.3533374
Produced by FastQC (version 0.11.2)