FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l02n01_r8.351000000a8acc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l02n01_r8.351000000a8acc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38936471
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGATGGCACATAGGACGTTAGGGTGGATAGCTTAGACGATGTCG394400.10129320656717966No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG299450.029.7686841
CCTATAC380150.026.2636343
TATAGGG318800.024.970822
CTATACT445900.024.713094
CCCTATA385400.023.6874732
TAGGGAT332450.023.188294
ATAGGGA329950.022.9177533
TCCCTAT401600.021.9838181
TACTAAC419100.020.6838957
TATACTA549250.020.5999725
AGGGATG385850.020.2015655
ATACTAA476700.019.6795026
CTAACCA473200.018.4596799
ATGGCAC401000.017.4361489
GACGTTA193350.017.19608320-21
TATACTG225850.016.615065
GGATGGC430450.016.4749557
GGGATGG459350.016.2968856
TAGACTG126750.016.0020225
CGTTAGG211150.015.5663322-23
Produced by FastQC (version 0.11.2)