FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l02n01_g3.351000000a8cbd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l02n01_g3.351000000a8cbd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49446458
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG164200.015.997245
TATACTG281400.015.2425185
GTATTGG384100.015.1668741
TACACCG179950.014.25394255
TACACTG214250.013.878635
TATACCG157550.013.778155
GTATAGG182850.013.5911631
GTGTAGG202200.013.3480261
GTCTATA215650.013.1985811
AGTACCG132150.013.1554895
GTACCGT127000.013.0156666
GTATAAG197550.012.9887281
CTATACT269900.012.9699774
TATACAG265750.012.95859055
GTACAGG199750.012.9170381
CCTATAC186450.012.7629313
TATTGGG411300.012.7146282
CTACACC280150.012.6988924
ACTGTGC219450.012.68382558
TACACAG222400.012.6438685