FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l01n02_r6.352000000a8ce1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l01n02_r6.352000000a8ce1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30677372
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG213000.028.0325531
TATAGGG211250.023.8336142
CCTATAC247750.023.2943443
CTATACT285800.021.8126034
CCCTATA239000.021.0067022
ATAGGGA225050.020.0086233
TAGGGAT246150.019.2455884
TCCCTAT255100.017.4103761
TATACTA367950.017.2528135
AGGGATG276900.017.160085
TACTAAC265500.016.6445487
GACGTTA117650.016.44893820-21
TAGACTG103900.016.4180345
ATGGCAC266950.015.9489359
TATACTG177900.015.5983685
ATACTAA310750.015.4288526
CTAACCA294550.015.2772269
ACGTTAG124400.014.98378420-21
CGTTAGG131700.014.53171522-23
TATACCG97500.014.3279795