FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l01n02_r5.352000000a8c77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l01n02_r5.352000000a8c77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36447326
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG201850.023.5107821
CCTATAC215600.018.991963
TATAGGG198550.018.9717772
CTATACT254200.018.8428464
TAGACTG123150.016.1676795
CCCTATA203700.015.8337392
TACACCG125500.015.789215
TATACTG201250.015.53667455
TAGGGAT220650.015.3979754
GTGTAGG174100.015.1161571
ATAGGGA220350.015.0255723
TATACTA334250.014.3587955
GTATTGG192250.014.1091261
GTATATA428750.013.9936051
ACTGTGC161700.013.9885518
GTATAGA172750.013.9143461
TACACTG154500.013.71748355
TACACAG156250.013.5030225
CCTACAC160900.013.3737573
GTGTATG225300.013.2834141
Produced by FastQC (version 0.11.2)