FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l01n02_r4.352000000a8bbb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l01n02_r4.352000000a8bbb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47221675
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG323950.026.731911
CCTATAC377850.022.9551493
TATAGGG318750.022.9192222
CTATACT428400.022.3162754
CCCTATA369600.020.447112
ATAGGGA351550.019.4704363
TAGGGAT362850.019.2239284
TATACTA552550.018.5492525
TCCCTAT398500.017.8568481
TACTAAC406000.017.2047487
ATACTAA469200.016.6191046
CTAACCA454850.016.2031949
AGGGATG440350.016.1213135
GACGTTA183500.015.72241620-21
TATACTG250700.015.0302665
TAGACTG154750.014.6772615
ATGGCAC426800.014.5849149
GTCCTAG166800.014.1540791
ACGTTAG204950.013.96105420-21
GTGTAGG218100.013.8523261
Produced by FastQC (version 0.11.2)