FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l01n02_r3.352000000a8b35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l01n02_r3.352000000a8b35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36665759
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG222950.026.228561
CCTATAC251400.021.0106323
CTATACT286450.020.6520374
TATAGGG224150.020.0680372
CCCTATA243950.018.1082212
TATACTA356100.016.9866035
ATAGGGA243650.016.395673
TATACTG193650.016.2193725
GTATTGG287250.016.2065261
TAGGGAT253750.016.0478614
TACACCG134600.015.3918755
TACACTG163900.015.3365195
GTGTAGG180350.015.2505781
TCCCTAT263500.014.9450651
TACACAG153600.014.8800355
TAGACTG120550.014.544855
TACTAAC269200.014.3507657
ATACTAA301650.014.1144596
GACGTTA117050.014.09924720-21
TCTATAC160150.014.0588883