FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l01n02_r2.352000000a8abc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l01n02_r2.352000000a8abc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34271779
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG164750.018.885761
GTATTGG235800.016.9825291
CCTATAC172800.016.3556213
TATACTG199500.016.1248465
TACACCG118650.016.099345
CTATACT221100.015.7313664
TAGACTG112000.015.6976095
GTGTAGG147850.015.454051
TATACCG111150.014.7916435
TACACTG164300.014.6629115
TGCGTTC170000.014.5068999
TACACAG146000.014.2876725
TATTGGG256500.013.7406482
GTGTAAG103750.013.5983721
GTCCTAG126100.013.4107631
CTACACC192200.013.3748224
GTATATA433100.013.3591161
TATACAG194750.013.2730655
ACTGTGC156600.013.2297278
GTATAAG147800.013.0166481
Produced by FastQC (version 0.11.2)