FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l01n02_r1.352000000a8a79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l01n02_r1.352000000a8a79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54817506
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG266300.019.2656211
CCTATAC272250.017.3779493
CTATACT331850.016.652924
TATACTG303600.015.3697435
TACACTG255400.014.9586375
TAGACTG177800.014.4050225
TACACAG239850.014.3831425
TACACCG187700.014.3287745
GTCCTAG193550.013.6216671
GTGTATG331150.013.5848751
TATACAG307200.013.5036115
GTATATA661100.013.4011521
ACTGTGC239300.013.38390358
GTATAAG223150.013.3475361
CTACACT246000.013.34736254
TATACCG168050.013.3462845
TAGACAG181500.013.2997665
GTGTAAG162350.013.1671161
GTCTAGG200250.012.9524091
CCTACAC243900.012.95140653