FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l01n01_r6.351000000a8ce4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l01n01_r6.351000000a8ce4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30677372
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG217150.028.7088761
TATAGGG209600.025.2211862
CCTATAC246100.023.2754083
CTATACT294450.022.2118134
ATAGGGA228550.021.2388653
CCCTATA240500.020.9537582
TAGGGAT235450.020.9190354
TATACTA385300.018.1703935
TCCCTAT256850.018.074121
AGGGATG277900.017.8263175
TACTAAC267750.017.224667
GACGTTA120400.017.17989320-21
TATACTG171850.016.7766425
ATACTAA317500.016.2312476
ACGTTAG127050.015.55168420-21
ATGGCAC277700.015.5470849
TAGACTG103650.015.4886395
CTAACCA302200.015.4811319
CGTTAGG129300.015.44637722-23
GGACGTT138350.014.79635618-19