FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l01n01_r5.351000000a8c7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l01n01_r5.351000000a8c7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36447326
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG201000.021.6559641
CCTATAC218050.020.1708053
CTATACT258100.019.76454
TATAGGG197900.016.8244382
TATACTG205050.016.8170575
CCCTATA203000.016.448552
TACACTG165900.016.2334125
TACACCG127550.015.565715
TAGACTG119200.015.5005265
TATACTA341750.015.2465385
TACACAG165850.015.235945
GTGTAGG160700.014.4009521
CTACACT172600.014.1721584
TATACCG113000.013.9971275
TAGGGAT220300.013.9064024
TATACAG208850.013.8956815
TCTATAC164400.013.7522413
ACTGTGC160800.013.6760768
GTATATA428900.013.6056851
CCTACAC165100.013.5788593
Produced by FastQC (version 0.11.2)