FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l01n01_r4.351000000a8bbe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l01n01_r4.351000000a8bbe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47221675
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG319250.026.1518881
CCTATAC384300.023.1738173
TATAGGG318700.022.0420932
CTATACT441350.021.9110034
CCCTATA368650.021.0654262
TAGGGAT358600.018.8478664
ATAGGGA349900.018.7734853
TCCCTAT402900.018.1747131
TATACTA575500.017.9840815
TACTAAC416950.017.5886977
ATACTAA487950.016.73316
CTAACCA470250.016.362769
AGGGATG440050.016.1475225
TATACTG251850.015.7666955
GACGTTA189750.014.91860420-21
TAGACTG149150.014.71279055
TACACCG154350.014.3402145
ATGGCAC421500.014.3337539
TACACTG210650.014.2956585
GTGTAGG206450.014.1093741
Produced by FastQC (version 0.11.2)