FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l01n01_r3.351000000a8b38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l01n01_r3.351000000a8b38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36665759
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG224100.025.7415731
CCTATAC249650.022.2026313
CTATACT292950.021.0934874
TATAGGG223050.020.357332
CCCTATA239800.019.3117052
TATACTG191700.017.6907635
ATAGGGA245350.017.1519243
TATACTA365250.016.8793225
TAGGGAT260050.016.8398674
TAGACTG122400.016.2981535
TACACCG134650.016.226395
GTATTGG290150.016.1805271
TCCCTAT264950.016.1591741
TACACTG162750.015.9930035
TACTAAC269950.015.4482087
ATACTAA303150.015.0725956
ACTGTGC161050.014.657598
CTAACCA297500.014.6243319
GACGTTA122550.014.51451420-21
CTACACT159700.014.4840034