FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l01n01_r2.351000000a8abf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l01n01_r2.351000000a8abf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34271779
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG169300.018.636711
CCTATAC167950.016.8549883
CTATACT216750.016.52254
TAGACTG115900.016.3925445
TACACTG159350.016.3640235
GTATTGG232550.016.2037261
TATACTG200850.016.1990135
TATACCG116150.015.4576135
TACACAG150400.015.1271655
GTGTAGG138150.014.996631
TATACAG197250.014.5923585
CTACACT161250.014.0207334
CCTACAC141300.013.9834193
GTATAGA175650.013.7968551
TGCGTTC172850.013.7393459
ACTGTGC155250.013.7366088
GTATTAG155150.013.6903271
TATTGGG257500.013.6679452
TAGACAG113550.013.5945895
TCTATAC167300.013.5420583
Produced by FastQC (version 0.11.2)