FastQCFastQC Report
Tue 21 Nov 2017
CAWVTACXX_l01n01_r1.351000000a8a7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWVTACXX_l01n01_r1.351000000a8a7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54817506
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG259750.020.0504461
CTATACT326500.018.0969664
CCTATAC272400.018.0294133
TATACTG305150.016.0634525
TACACTG250850.014.9962835
TACACAG237000.014.79042155
TAGACTG178650.014.676035
TACACCG192500.014.4343635
ACTGTGC236350.014.3686598
TATACCG167000.014.1924245
TATACAG318300.013.8777425
GTGTAGG234100.013.6610071
TATACTA478050.013.4231545
GTCCTAG197250.013.0331471
TAGACAG176450.013.0285545
TCTATAC244400.012.9820553
GTGTATG337950.012.9783151
ACAGTGC273400.012.9426598
CTACACT249200.012.8273114
CCCTATA259550.012.7366842