FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l08n02_ogla_9.3520000009baf7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l08n02_ogla_9.3520000009baf7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31127106
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG147850.017.5873971
TATACTG199450.016.9647245
TAGACTG99200.016.4796355
GTATTGG285500.016.2176691
TACACTG142950.016.156715
CCTAGAC103100.015.6290023
CTATACT204900.015.4490914
GTCTAGG109700.015.3835761
CCTATAC141500.015.28428653
GTATAAG146650.015.2353121
GTGTAGG130050.014.9868061
TATACAG189150.014.6726075
TACACCG101850.014.4644365
GGTGCGT207600.014.2531557
TGCGTTC198800.014.1910579
TCTATAC161100.014.0443493
GTATATA431700.014.039891
TATACCG109100.014.0259395
CTACACT137650.013.984584
CCTACAC126700.013.9183423