FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l08n02_ogla_14.3520000009bd8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l08n02_ogla_14.3520000009bd8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18717106
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG86250.017.3611181
TATACTG122000.016.6714425
GTATAAG84400.015.3197851
CTATACT120950.015.1684194
GTATTGG149350.015.0550481
TATACCG62550.014.9667725
TCTATAC102950.014.9588583
CCTATAC84250.014.83766653
GTATATA264250.014.4632961
TATACAG112750.014.4144745
ACTGTGC88400.014.3987428
GTATAGA97500.014.13902951
GTGTAGG82400.013.7301721
TACACCG64200.013.545825
GTCTAGG72050.013.4592981
TACACTG91000.013.0553285
ATACTGT124650.012.9929446
GTATTAG100100.012.9169821
TACACAG86900.012.7963265
TAATACT147950.012.7215214