FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l08n02_ogla_13.3520000009bd03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l08n02_ogla_13.3520000009bd03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26395215
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG122050.016.903921
TATACTG173550.016.5938685
GTATTGG255200.016.2059351
GTATAAG130950.015.06531051
TACACTG117450.015.0113775
TATACCG91250.013.9573045
CCTATAC118550.013.8727153
CTATACT170000.013.7837824
TGCGTTC176300.013.7389699
TATTGGG281950.013.7233922
TAGACTG86700.013.7031555
GTGCGTT188500.013.5805128
GGTGCGT188200.013.5517917
GTACTAG194500.013.4668961
GTATATA386350.013.37470151
GTCTAGG96150.013.2995971
ACTGTGC112900.013.2091388
GTATTAG134350.013.1625721
GTATAGA149700.013.1466181
TCTATAC144850.013.0930643
Produced by FastQC (version 0.11.2)