FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l08n02_ogla_12.3520000009bca5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l08n02_ogla_12.3520000009bca5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21820103
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG142100.016.954955
GTATAGG103050.015.7295581
GTATTGG199250.015.697761
TATACCG70350.015.4011595
GTCTAGG82550.014.7987881
GTATAAG101450.014.75939751
GTGTAGG95850.014.6298551
CTATACT148350.014.48125654
TACACCG73850.014.4138565
ACTGTGC104850.014.3594088
TAGACAG83350.014.3103675
GTATATA309500.014.14521
CCTATAC102500.014.1432193
TAGACTG70350.014.050185
TACACAG102200.013.9958175
CCTAGAC78850.013.9848753
TACACTG104050.013.8383155
TAGACGG36950.013.7610645
TTATACT170750.013.4165764
TATACAG130300.013.3845665