FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l08n02_ogla_11.3520000009bc2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l08n02_ogla_11.3520000009bc2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39891706
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG258650.017.0321035
GTATTGG352750.016.0233211
GTATAAG191050.015.9495591
TAGACTG131900.015.7808475
GTATAGG187100.015.6764991
TACACCG135050.014.9553065
CCTATAC183600.014.8604043
CTATACT268750.014.7500214
GTCTAGG149900.014.7147891
TATACCG126950.014.6367625
TACACTG192700.014.4762935
GTGTAGG165000.014.3765391
GTATATA559600.014.3309541
CCTAGAC134200.014.23852353
TATACAG236350.013.6525945
GTATAGA220900.013.49303151
TATTGGG404600.013.3817262
GGTGCGT263850.013.3206077
CTACACT189950.013.1870124
GTATTAG204250.013.1732241