FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l08n02_ogla_10.3520000009bb7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l08n02_ogla_10.3520000009bb7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32467956
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG273850.016.6819361
TATACTG217300.016.4674055
TACACCG107750.016.0095565
GTATAGG146350.015.9161931
TATACCG103700.015.03095
TACACTG158350.015.0052025
TATACAG192950.014.7527155
TGCGTTC191000.014.7205729
CCTAGAC107450.014.6864883
CTATACT214750.014.6518244
TAGACTG101900.014.5502455
GTGTAGG136800.014.4558321
GTCTAGG119950.014.26717951
GTATATA477600.014.2134581
GTGCGTT203600.014.1129338
GTATAAG144500.014.0144991
CCTATAC144950.013.8710223
TATTGGG299750.013.8442512
GGTGCGT210950.013.8014267
GTATAGA189300.013.7363871