FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l08n01_ogla_9.3510000009bafa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l08n01_ogla_9.3510000009bafa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31127106
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG200950.018.866945
GTATTGG277750.016.7998241
TACACTG141800.016.1494375
CTATACT211400.015.9790594
CCTATAC141250.015.7077873
GTATAGG143100.015.6065011
TATACCG99200.015.5653285
TAGACTG102350.015.4576335
TACACCG103250.015.1848495
GTGTAGG129650.014.7333271
TGCGTTC196150.013.9954189
TCTATAC170500.013.9883313
TATTGGG311750.013.8987252
GTATAAG150800.013.8328121
TATACAG181350.013.8325725
ACTGTGC137450.013.8217048
GTATATA441350.013.8130881
GGTGCGT212500.013.8126147
TACACAG139000.013.774535
CCTAGAC101550.013.7080053
Produced by FastQC (version 0.11.2)