FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l08n01_ogla_8.3510000009ba9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l08n01_ogla_8.3510000009ba9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39611301
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG269500.017.2589155
GTATAGG184200.014.9360791
GTATAAG197450.014.7038741
GTATTGG338600.014.285931
TATACCG129050.013.9531025
GTATATA597700.013.8170151
TTATACT318000.013.7451744
CTATACT264900.013.5949244
TACACTG183850.013.2052565
TATACAG242950.012.9458525
TAATACT339400.012.8225154
ACTGTGC178350.012.6227468
GTATTAT315050.012.6088211
CCTATAC179650.012.4561343
TAGACTG131350.012.2257685
TCTATAC215500.012.2138353
ATTATAC297200.012.1174143
TAGACAG148150.012.0900825
GTATAGA231650.012.0817751
GTGTAGG161000.011.8349441
Produced by FastQC (version 0.11.2)