FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l08n01_ogla_14.3510000009bd8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l08n01_ogla_14.3510000009bd8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18717106
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG120050.017.4124935
TACACTG89100.015.4628925
GTATAAG86450.015.3892691
GTATAGG85850.014.9987121
TATACCG59750.014.8687395
GTATTGG149050.014.3132861
TATACAG115250.014.1803825
TAGACTG59250.014.1122015
TTATACT144450.013.9778964
CCTAGAC62450.013.92159753
GTATTAG99300.013.4456581
ACTGTGC85050.013.4023488
GTATATA266950.013.3492341
CTATACT126700.013.3113524
CCTATAC87550.013.2947553
TACACCG63650.013.2859335
GTATTAT139200.013.2780821
GTGTAGG82900.012.7239931
ACAGTGC95750.012.6982878
ATACTGT129550.012.648116