FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l08n01_ogla_12.3510000009bca8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l08n01_ogla_12.3510000009bca8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21820103
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG147850.016.6126775
GTATAGG104450.016.5130121
GTATTGG195000.015.7407721
GTATAAG108450.014.9838931
CTATACT150150.014.8078144
TACACCG80250.014.5041225
TACACTG100550.014.4580515
CCTAGAC74750.014.236633
TCTATAC115200.014.1865363
TAGACTG75300.013.9433685
TACACAG93300.013.850255
GTGTAGG96650.013.6669331
TAGACAG84150.013.5496325
TATACAG135950.013.3491775
GTATATA317600.013.3448121
GTGTAAG67100.013.2418061
GTGTATG148500.013.1185411
TATTGGG221000.013.0916812
GTCTAGG88300.013.0221531
CTACACT109450.012.9351324
Produced by FastQC (version 0.11.2)