FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l08n01_ogla_11.3510000009bc21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l08n01_ogla_11.3510000009bc21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39891706
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG264650.017.6643945
GTATTGG351750.016.209861
GTATAGG185200.016.0350841
TATACCG130100.015.3007345
TAGACTG131450.015.0351685
CCTATAC183950.014.9022393
TACACTG184800.014.8079875
TACACAG178800.014.7469095
GTGTAGG165450.014.589121
CTATACT274900.014.4479774
TATACAG244600.014.1983045
GTATAAG189700.014.0516621
TCTATAC211850.014.0160953
TACACCG137700.013.4901995
GTCTAGG146550.013.422911
GTATTAG203800.013.4058951
GTATATA562200.013.26063251
GTATAGA224550.013.2462641
TAGACAG150200.013.1899045
TATTGGG403300.013.1347612