FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l07n02_ogla_7.3520000009bdda.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l07n02_ogla_7.3520000009bdda.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42944094
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG272950.016.3126225
GTATAAG211450.015.6229031
GTATAGG196700.015.6103551
TATACCG149050.014.0915115
GTATATA614250.013.6192181
GTATTGG388450.013.6067481
TAGACTG137400.013.142085
TACACTG198550.013.0913575
TTATACT344650.013.0445234
CTATACT294500.012.8291294
TACACCG131900.012.8254455
TCTATAC232200.012.7722993
CCTATAC202600.012.7381713
GTGTAGG167300.012.6997811
ACTGTGC190150.012.6642258
GTATTAG235400.012.4584311
TATACAG258300.012.4546515
GTATTAT351150.012.3313441
GTGTATG278350.012.1924681
GTATATG417800.012.1616911