FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l07n02_ogla_6.3520000009bcf5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l07n02_ogla_6.3520000009bcf5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41527108
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG278250.016.2755325
GTATTGG372500.016.0016731
TATACCG141050.015.8007315
GTATAGG188450.015.5878571
TATACAG253100.015.0952855
CCTATAC192600.014.2388843
GTATAAG199950.014.073251
GTATTAG224900.014.0337181
CTATACT287050.013.9244
TACACTG194250.013.91966155
GTATAGA241250.013.8313281
TAGACTG138950.013.5429745
GTATATA610250.013.5140581
ACTGTGC187550.013.4223188
GTGTAGG171150.013.41418651
TGCGTTC254950.013.3202189
TTATACT341950.013.1617294
TACACCG130850.013.1102485
GGTGCGT276100.013.0055997
GTGCGTT276900.012.984998