FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l07n02_ogla_5.3520000009bc8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l07n02_ogla_5.3520000009bc8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38470333
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG249800.016.8929215
TATACCG135200.016.2386365
GTATAGG178800.015.7381911
GTATAAG186800.015.5731031
GTATATA527200.014.4530241
CCTATAC182350.014.283673
GTATTGG318450.014.1205141
TAGACTG124300.013.9542325
ACTGTGC166900.013.6885458
TAGACGG63200.013.60961155
CCTAGAC129500.013.5065073
GTCTAGG148250.013.4985771
TATACAG232550.013.4664565
TACACTG174850.013.4531445
GTGTAGG153150.013.439141
CTATACT257450.013.3837374
TACACCG121950.013.0541095
TAGACAG151250.012.9445235
GTATTAG200400.012.9270491
GTATATG367050.012.7170631