FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l07n02_ogla_4.3520000009bc04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l07n02_ogla_4.3520000009bc04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28986698
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG133500.016.0572911
GTATTGG251850.015.5322681
TATACTG187400.015.3153935
TACACTG137650.014.5679035
GTGTAGG125550.014.38612651
GTATATA400500.014.2414991
GTATAGA161500.013.9208451
TATACCG93500.013.8743235
CCTATAC135550.013.7795423
TACACCG99200.013.747735
TAGACAG107350.013.4565065
CTATACT191950.013.394284
TGCGTTC169400.013.3180139
GTGTAAG91850.013.2993671
CCTAGAC101900.013.2926763
TATACAG169050.013.2112365
GTCTAGG106200.013.2030571
TAGACTG94950.013.0618995
GTATAAG133000.012.6868481
GTATTAG154050.012.6502511
Produced by FastQC (version 0.11.2)