FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l07n02_ogla_3.3520000009bb8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l07n02_ogla_3.3520000009bb8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30854957
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG199150.017.1326795
GTATAGG137600.017.0995541
GTATTGG266400.016.2548221
TATACCG97700.015.515915
GTCTAGG118550.015.316511
GTATAAG142900.015.2346081
CCTATAC142750.015.1161743
TACACCG105850.015.0844525
TACACTG144650.014.8819865
GTGTAGG130600.014.3400641
CTATACT202350.014.3034794
ACTGTGC138350.014.0415878
TGCGTTC180150.013.9998439
TAGACTG100950.013.7454085
GTGCGTT196300.013.5742118
TATACAG182300.013.528885
GGTGCGT202000.013.473457
GTATATA436250.013.4455251
CCTAGAC106050.013.2660963
GTACTAG229450.013.2583641