FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l07n02_ogla_2.3520000009bb05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l07n02_ogla_2.3520000009bb05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32613269
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG286300.016.5196631
TATACTG208950.015.9426025
TATACCG104700.015.6133225
TACACCG107800.015.340665
TACACTG155050.015.0178535
GTATAGG146850.014.7925061
GTATAAG143250.014.4674281
GTGTAGG144900.014.3354891
GTCTAGG115650.014.3031961
TATTGGG318100.014.11992
GTATAGA175100.013.9804321
TAGACTG102000.013.7903425
CCTATAC150800.013.7419383
CTACACT150600.013.6961014
GTATATA459900.013.6119971
GTATTAG167000.013.5199971
TATACAG186100.013.4825215
ACTGTGC146050.013.3988068
CTATACT213700.013.1436254
GTGTATG212100.013.0655391