FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l07n02_ogla_1.3520000009ba8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l07n02_ogla_1.3520000009ba8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47696187
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG304600.016.4896665
GTATAGG221050.016.126971
GTATTGG404800.015.1119351
TATACCG161700.014.6347775
TAGACTG147800.013.760565
GTATAAG234350.013.7513951
GTGTAGG189500.013.56969451
GTCTAGG176550.013.4881391
TACACTG212850.013.3950515
GTATATA661850.013.3937231
TGCGTTC271050.012.8619459
GTATAGA280250.012.8559921
CCTATAC224700.012.7543933
GGTGCGT288550.012.7406677
GTGCGTT287550.012.5535658
ACTGTGC211150.012.484438
CTATACT318350.012.31580454
CTACACT212550.012.1856614
ATACTGT308600.012.0674536
TCTATAC253250.012.0672163