FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l07n01_ogla_5.3510000009bc8e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l07n01_ogla_5.3510000009bc8e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38470333
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG248650.017.1750095
TATACCG132700.016.466935
GTATAGG183550.014.9602261
CCTAGAC133500.014.6604333
GTATAAG192050.014.5454681
TAGACTG122150.014.5446695
CTATACT262400.014.0482954
GTATATA527300.013.8928481
GTGTAGG155650.013.7349821
TTATACT302100.013.6959824
TAGACAG148350.013.6730715
CCTATAC179600.013.6481623
GTCTAGG154250.013.42845251
GTATAGA224050.013.4009881
TCTATAC205600.013.2623233
GTATTGG313000.013.1898591
TATACAG225400.013.0034035
ACTGTGC169400.012.8410828
TACACTG167100.012.6789755
GTATTAG202600.012.5686911