FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l07n01_ogla_4.3510000009bc07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l07n01_ogla_4.3510000009bc07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28986698
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG191550.016.2431955
GTATTGG250050.016.015841
TAGACTG92650.015.17604355
TATACCG92900.015.1352055
GTATAGG136350.014.3894411
GTATAAG134700.014.3540951
GTGTAGG124500.014.1945811
CTATACT189500.014.0626114
TACACTG135100.013.9236195
TACACCG95950.013.91148955
TACACAG133050.013.8525315
GTATAGA160750.013.5351641
CCTAGAC99400.013.4765993
GTATATA400450.013.251161
TATACAG171200.013.12410455
ACTGTGC129350.012.998118
TCTATAC154150.012.8810213
CCTATAC132800.012.8414463
ACAGTGC140300.012.7960968
GTCTAGG104600.012.7621441