FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l07n01_ogla_3.3510000009bb8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l07n01_ogla_3.3510000009bb8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30854957
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG197200.016.9587145
GTATAGG141750.015.9537891
TACACTG144800.015.7470725
GTATTGG271950.015.2162991
TAGACTG99950.015.161255
CCTAGAC104350.014.8863453
TATACCG97100.014.87241655
ACTGTGC138250.014.8067758
TATACAG179350.014.59415
CTACACT142200.014.3646824
GTATAAG141600.014.2930951
CTATACT201450.013.9834574
GTGTAGG135250.013.840091
TACACCG104600.013.8060385
TAGACAG110900.013.7499355
CCTACAC134400.013.6433453
GTATATA446250.013.4463711
TGCGTTC188300.013.4186749
CCTATAC144800.013.3851853
TACACAG139800.013.1501575