FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l07n01_ogla_2.3510000009bb08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l07n01_ogla_2.3510000009bb08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32613269
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG211200.016.8242665
GTATTGG284100.016.7726671
TACACTG148350.015.9787015
GTATAGG140950.015.5047641
TAGACTG108450.014.9804545
CCTATAC144100.014.5051573
TATACAG186900.014.4366865
CTATACT208200.014.328724
TATACCG106600.014.170935
TACACCG111150.014.1036965
GTATAAG145500.013.9423921
TATTGGG323100.013.7617182
TACACAG144150.013.7419975
GTGTAGG139800.013.7292421
GTATATA457950.013.7250581
CCTAGAC104400.013.2866693
GTATTAG170200.013.1193021
GTCTAGG118800.013.11685851
CCTACAC138700.012.9121533
CTACACT151350.012.8371724