FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l06n02_ogla_45.3520000009ba70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l06n02_ogla_45.3520000009ba70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31616863
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG197750.015.2859645
GTATAGG139100.015.0373011
TATACCG99800.014.7632985
GTATATA416700.014.0892471
GTATAAG146550.013.9809211
GTATTGG291500.013.9108871
TATACAG181100.013.6994875
TAGACTG100800.013.6266625
GTATTAG157450.013.3149711
GTATAGA170800.013.3040661
GTCTAGG110350.013.1823671
CCTATAC141450.012.8701353
CTATACT197750.012.5228254
CCTAGAC108100.012.4876283
TTATACT233500.012.4782784
GTGTAGG133250.012.4509521
ACTGTGC139400.012.3335118
TAATACT247650.012.2835164
ATAATAC229800.011.9347433
TACACCG105550.011.8877025