FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l06n02_ogla_44.3520000009badd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l06n02_ogla_44.3520000009badd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28372401
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG181450.016.4755365
GTATAGG131800.015.2566951
GTATTGG243550.014.6779441
TATACCG91800.014.3411195
CTATACT184850.014.01357654
TAGACTG91750.013.6755165
GTATTAT214500.013.4744741
TATACAG160600.013.4355745
GTATAAG138700.013.2981361
GTATATA387400.013.1912031
GTATAGA156250.013.1735671
TTATACT222500.012.9453744
GTATAAT199950.012.7669861
TACACTG123650.012.7611515
ATTATAC207350.012.5619673
CCTATAC126150.012.39623453
ACTGTGC127350.012.232748
GTGTAGG117700.012.0761991
TATTGGG277600.012.020862
ATACTGT185100.012.0085086
Produced by FastQC (version 0.11.2)