FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l06n02_ogla_41.3520000009bbe9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l06n02_ogla_41.3520000009bbe9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25535953
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG165050.015.0318845
TATACCG84500.014.9055385
GTATATA359450.014.6934941
GTATAGG117700.014.3788151
GTATTGG226100.014.2343491
GTATAAG127550.013.34295751
GTATTAG135250.013.0402581
CTATACT161850.013.0099574
TATACAG147050.012.6700845
GTATTAT202350.012.5454871
TAGACTG82450.012.393855
GTATAGA143350.012.3034171
ACTGTGC112400.012.2962088
CCTAGAC82750.012.2924433
TACACTG118350.012.288865
CCTATAC112050.012.0899783
TTATACT203150.011.8858914
GTATTAA186950.011.8243431
TCTATAC135850.011.756343
GTCCTAG94700.011.7466881
Produced by FastQC (version 0.11.2)