FastQCFastQC Report
Sun 3 Sep 2017
CAWHCACXX_l06n02_ogla_40.3520000009bc61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAWHCACXX_l06n02_ogla_40.3520000009bc61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25908432
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG157600.015.9531395
GTATATA338650.014.556741
GTATAGG112700.014.0039521
GTATTGG236100.013.8726581
TATACCG87300.013.7193335
GTATAAG122400.013.632081
CTATACT161700.013.1980024
CCTATAC116400.012.9854223
TCTATAC129750.012.931513
TACACTG117200.012.8957385
GTATTAT193950.012.8433391
GTATTAA172900.012.75731851
TAGACTG83000.012.7122255
TATACAG146650.012.6718895
GTATAGA141000.012.6429551
GTGTAGG103300.012.4711191
GTATAAT192350.012.1593231
ACTGTGC110950.012.1143878
TAATACT202600.012.1054824
TACACAG112550.011.9927715